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The structure of th 5S rRNA isolated from Xanthomonas citri was determined by enzymatic and chemical degradation methods. It consists of 119 nucleotides and contains no modified nucleosides. As does the 5S rRNA of X. maltophilia, it contains an additional uridine residue on the 5'-terminus. The secondary structure of the 5S rRNA from X. citri was almost identical to the generalized models proposed by many other workers [De Wachter et al., Biochimie, 64, 311 (1982); Specht et al., Nucleic Acids Res., 18, 2215 (1990); Cho et al., Proceedings of the First Symposium on Biomolecules, p. 9 (1991)]. The secondary structure of 5S rRNA from X. citri consists of five helices, five loops and two bulges. This 5S rRNA has a uridine residue at position 66 as a bulge.
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